FastQCFastQC Report
Tue 4 May 2021
TC4_F3_fPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F3_fPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41531712
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA2251130.5420267770324517Illumina Single End PCR Primer 1 (100% over 52bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG1314880.31659662861959553No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT760050.18300473623625244Illumina Single End PCR Primer 1 (100% over 52bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA554190.13343779326987532No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC462930.11146422280882617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)