FastQCFastQC Report
Tue 4 May 2021
TC4_F1_fPb_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_F1_fPb_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32646368
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT3262410.9993179026836921Illumina Single End PCR Primer 1 (100% over 52bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA1895590.580643457795979Illumina Single End PCR Primer 1 (100% over 52bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG701860.2149886933823695No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA425280.1302687024786341No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA415350.1272270164938409No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC386310.11833169313045788No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC330040.10109547254996329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)