FastQCFastQC Report
Tue 4 May 2021
TC4_E2_fDEHP_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_E2_fDEHP_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40363850
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA1754530.43467855519233173No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT1678520.4158473485557002No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG1535690.38046172503366255No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT969080.2400861166613195No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT846690.20976443030087566No Hit
GTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCG800590.198343319579277No Hit
GCAGCACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGG589850.14613323555607308No Hit
GTCCTTGTCAGCAGCCTTGCAACATGTATCCAGGAACTGTGCAAAGTCATCC580530.14382423876810563No Hit
CCTCAGTCGAGAAGCAGGTGTCCTTGTCAGCAGCCTTGCAACATGTATCCAG550960.136498376641475No Hit
CTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCATG548760.13595333448122515No Hit
CACAGAGACAAGAAGTCAAATGTTTAAAGAAATTTGTGTTCCTTAGGGTGTT539940.1337682109114963No Hit
GATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTC537480.1331587546777624No Hit
GTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGACCC532860.13201416614123776No Hit
GTCTTCAGTTGCTCCGCTGTAGCCTTGGGCTTGTGCTTCACCAGCTCAGCAA528710.1309860184298574No Hit
GCCTTGTCTAAATTCTCTTGCTGACTCTCTTGTAGACAATGTCGCCCAATGT482670.11957977249444737No Hit
GTGTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTC480850.11912887398005888No Hit
GTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAAATCTCATTCAGGG461630.1143671874709672No Hit
GGCGTCTTTGCATCTAGTGACAAGGTTTGGACCCTCAGTCGAGAAGCAGGTG421590.10444742015442035No Hit
GTGGTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTT413650.10248031344879142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)