FastQCFastQC Report
Tue 4 May 2021
TC4_D1_fCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_D1_fCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35784734
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC599410.16750438888270067No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT583720.16311983763802743No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG582340.1627341983316126No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA474210.13251740253259953No Hit
CCTAAACACATCACAACCACAACCTTCTCAGGCTACCCTGAGAAAAAAAGAC408770.11423027484289809No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA408110.11404583865287359No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC388380.10853231436623226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)