FastQCFastQC Report
Tue 4 May 2021
TC4_B6_mDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B6_mDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27913905
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA8087882.89743767487924Illumina Single End PCR Primer 1 (100% over 52bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT4743391.6992928793015525Illumina Single End PCR Primer 1 (100% over 52bp)
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC705140.25261245246768593No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT580990.20813641086763032No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC575880.20630578201079355No Hit
CCTAAACACATCACAACCACAACCTTCTCAGGCTACCCTGAGAAAAAAAGAC532030.19059676530388708No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA503160.18025424962935138No Hit
GTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCT452640.16215574280990067No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG419200.15017605025165773No Hit
CTTCACCTTCCACTCTGATATCTGCACACTTCCAGAGAAGGAGAAGCAGATT391200.1401452072004974No Hit
AGGACACCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGA337130.1207749327799174No Hit
GACAGATATGACCAGTCAAAGTGCAAGACTACCTACCCACTGCCATGAAAAC325230.11651182448317425No Hit
CCTGGATACATGTTGCAAGGCTGCTGACAAGGACACCTGCTTCTCGACTGAG323430.11586698457274251No Hit
CACAAGCCCAAGGCTACAGCGGAGCAACTGAAGACTGTCATGGATGACTTTG299030.10712582134244564No Hit
GACAAGGACACCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCA297780.10667801584909027No Hit
GTTTAGTCTACATTGCTCTTCCCAGAGACTGACAGATATGACCAGTCAAAGT287070.10284121838202143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)