FastQCFastQC Report
Tue 4 May 2021
TC4_B2_mDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B2_mDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30618905
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA770950.25178888663719357No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC666790.2177706877499375No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATC651480.21277050893884022No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT549460.17945122466005886No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG534050.17441838628781794No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC511080.16691648509311485No Hit
AGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTG481950.15740275493196115No Hit
GGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATC472750.15439807530674268No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCA447800.14624951480139475No Hit
AGGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATAT434430.14188293147648487No Hit
AGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAA432080.14111543178960842No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCC430830.14070718727531242No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTAT429370.14023035768261471No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC388300.12681707592090571No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG382970.12507632131194762No Hit
GCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGC378700.12368175805111253No Hit
CACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGATC373740.12206184381838607No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA370340.12095141873950098No Hit
CTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATCCCCAG354020.11562137836085254No Hit
GTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCA342140.11174142249698348No Hit
GAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTCC328910.10742056255767475No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC325500.10630687152267529No Hit
CAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCC316140.10324993659962695No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT315000.102877617602589No Hit
GGAAAGGTTTGCACAACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATC314940.10285802186590279No Hit
GGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT311360.10168880957695907No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT307760.10051306537578664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)