FastQCFastQC Report
Tue 4 May 2021
TC4_B2_mDEHP_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_B2_mDEHP_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30618905
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA1099530.3591016726430942No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC969180.31652993469230856No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA892120.2913624768749895No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT842400.2751241430743523No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG759460.24803630306178487No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT600520.19612719658002137No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC527080.1721420148761035No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATAT486300.1588234458417112No Hit
GGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCT459530.15008048132354831No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG443800.1449431323556476No Hit
GGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGG418250.1365986144834376No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGA402880.13157883993565414No Hit
CCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTTCAT398220.1300569043863587No Hit
CCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATG377440.12327024758070218No Hit
GGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGG375190.1225354074549694No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATA363470.11870770688893022No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGG354890.11590551654280257No Hit
GGTGTATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCT349030.11399166625978296No Hit
AGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGA337970.11037951879729208No Hit
GTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCA331700.10833176431358339No Hit
GGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACA328400.10725399879584199No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACT327490.1069567967894345No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTC319890.1044746701425149No Hit
CTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG308270.1006796291376194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)