FastQCFastQC Report
Tue 4 May 2021
TC4_A6_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A6_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28453193
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT532270.18706863584695047Illumina Single End PCR Primer 1 (100% over 52bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA504840.17742824153338432Illumina Single End PCR Primer 1 (100% over 52bp)
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA430060.1511464811699692No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC421940.14829267140598246No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC377330.1326142904242768No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT359270.12626702388023728No Hit
GGATGACTTTGCACAGTTCCTGGATACATGTTGCAAGGCTGCTGACAAGGAC319200.11218424589465233No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT316940.11138995894063629No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA307950.1082303838447938No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG306490.10771726041432328No Hit
GGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATC300660.10566828123648549No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC292150.10267740425477029No Hit
CCTAAACACATCACAACCACAACCTTCTCAGGCTACCCTGAGAAAAAAAGAC292000.1026246860941055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)