FastQCFastQC Report
Tue 4 May 2021
TC4_A4_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A4_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45570978
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA1043810.22905148096668015No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTC954250.20939862207916626No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTGGA860860.1889053160105539No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATAT784090.17205906794451503No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATT563230.12359401196085806No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGC544040.11938299853911409No Hit
CCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGTTG543680.11930400089284895No Hit
GTCCTGAGTCTTCATGTCTTTTTTTCTCAGGGTAGCCTGAGAAGGTTGTGGT528980.11607826367035616No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC528090.11588296393375626No Hit
GTTCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT523770.11493499217857471No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTC522720.11470458237696807No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT515470.11311365755635087No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATG491030.10775059512657376No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT477290.10473551829412132No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTA467570.10260258184496282No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC464880.10201229387703727No Hit
GGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCT462540.10149880917631393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)