FastQCFastQC Report
Tue 4 May 2021
TC4_A3_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A3_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39003423
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC884630.2268082983383279No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA856000.21946791695692966No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC822970.21099942946033223No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT787680.201951505640928No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCA762690.1955443756821036No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTAT710140.18207119923807713No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATG704760.1806918331244927No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC702780.18018418537265307No Hit
GGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATC620900.1591911561198103No Hit
AGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTG566860.14533596192313683No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT541880.13893139584184702No Hit
CCACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGAT540220.13850579217111278No Hit
GGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATC521000.13357801954972004No Hit
CACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGATC512130.13130386017657988No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCA505510.12960657324871203No Hit
AGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAA488940.12535822817397332No Hit
CTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCC478400.12265590125256443No Hit
CAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCC475290.12185853533932137No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCTCC467520.11986640249498101No Hit
CTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATCCCCAG462050.11846396148358568No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTA460170.11798195250709148No Hit
AGGAAAGGTTTGCACAACTATGTGAGGAGCATGGAATCCTTAGAGAAAATAT458320.11750763516320092No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC441780.11326698172106588No Hit
GAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTCC434990.11152610887511077No Hit
GTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCA423000.10845201971119303No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT420110.10771105910370996No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA400340.10264227321791732No Hit
AGCAGATTAAGAAACAAACGGCTCTTGCTGAGCTGGTGAAGCACAAGCCCAA395250.10133725955283462No Hit
CCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACC395060.1012885458796783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)