FastQCFastQC Report
Tue 4 May 2021
TC4_A2_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC4_A2_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37520861
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG857330.22849422352008392No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA548440.14616935362970482No Hit
CTTGTCACTAGATGCAAAGACGCCTTAGCCTAAACACATCACAACCACAACC541910.14442898844991856No Hit
TGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACTC513110.1367532584073697No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCA478020.12740112760205582No Hit
CCTGCTTCTCGACTGAGGGTCCAAACCTTGTCACTAGATGCAAAGACGCCTT473790.12627375475205646No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCA460880.12283300215312222No Hit
CCAGTGTTGAGTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTAT414830.1105598296371717No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTC385440.10272685373611229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)