FastQCFastQC Report
Tue 4 May 2021
TC5_E5_fDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_E5_fDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66247653
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT2787340.42074547154145975No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1628420.24580795337760872No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCG1057650.15965093887929885No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGG927630.14002458321051767No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG856130.1292317480288698No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA821380.12398627918184513No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAG815550.12310624800549538No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA710880.1073064429920257No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA703420.10618036536328312No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGG684690.10335309539192278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG679820.10261797500962033No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTC676130.10206097414500104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)