FastQCFastQC Report
Tue 4 May 2021
TC5_D3_fCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_D3_fCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60615684
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG3357420.5538863506019333TruSeq Adapter, Index 3 (97% over 36bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC1892250.31217168150738017No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGC1286990.21231963661418057No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC1270860.20965860914808782No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC935490.15433134434315712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATG905250.14934253649599996TruSeq Adapter, Index 3 (97% over 36bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC877000.14468202651973702No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA844880.13938306792017724No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA736870.12156424729942832No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA717070.11829776597093253No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG714490.11787213355540127No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACC673410.1110950096677949No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTA673040.11103396936014119No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT634390.10465773181739566No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG622610.10271434040074513No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG621530.10253616869191809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)