FastQCFastQC Report
Tue 4 May 2021
TC5_B2_mDEHP_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_B2_mDEHP_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54289867
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT2069070.38111531936521414No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1120340.20636263485412482No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCG755620.1391825107989305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG755580.13917514294149957No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGG658010.12120309670311036No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG632230.11645451258887778No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAG557430.1026766191930439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)