FastQCFastQC Report
Tue 4 May 2021
TC5_A2_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC5_A2_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57762564
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT2772080.47990944446302625No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1499940.25967337599487444No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCG1020120.17660573377594527No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGG880450.1524257129583098No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG874240.1513506221780598No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA748140.12951987380615582No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAG738820.12790637202323638No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA735180.1272762060908515No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGG661280.11448245268336772No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA648070.11219550434083916No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTC597660.10346839866734447No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT596400.10325026430613433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)