FastQCFastQC Report
Thu 7 Jan 2021
TT_BPA22_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTT_BPA22_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53015131
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG1666380.3143215849075238No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG1092240.2060242009021915No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTG1010850.19067198004282968No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTG610170.11509355696961306No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCGGCTCTGCTTTTTCCA578950.10920467215293687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)