FastQCFastQC Report
Mon 8 Mar 2021
TC8_A7_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A7_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28547265
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3346651.1723189594519825Illumina Single End PCR Primer 1 (100% over 50bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2517020.8817026779973492No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1485490.5203615827996132Illumina Single End PCR Primer 1 (100% over 50bp)
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC1409600.4937776000608114No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC976570.34208881306142636No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA918410.3217155829113577No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT848540.2972403836234399No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC765700.2682218419172555No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG686630.24052391708978077No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA646620.22650856395525104No Hit
CTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGC582770.2041421481182173No Hit
CTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGA481980.16883578864735377No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA446520.15641428346988756No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT418810.14670757426324377No Hit
GGCACCATCACCAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTAT368480.1290771637843415No Hit
CTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATT319590.11195117991163075No Hit
GGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAA300410.10523249775416313No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG300050.10510639110261526No Hit
CAGAAAGACGTGGTTCTGACAGGCAGAAAATTTTACTATCTACCAAAATG290850.10188366556305832No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC290180.10164896707267754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)