FastQCFastQC Report
Mon 31 Oct 2016
G142-M7-Index-19_GTGAAA_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M7-Index-19_GTGAAA_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22668814
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC878530.3875500500379067No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG616510.27196394129838464Illumina Single End PCR Primer 1 (100% over 50bp)
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC530860.23418075599367486No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC491700.2169059219419243No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC442110.1950300531823147No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC435920.1922994295158097No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC361710.15956282494531915No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC352810.15563672629719402No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG270720.11942398045173426No Hit
CGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCG235410.1038475149163075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG96500.035.30153735
GATCTCG97050.035.11653534
CGTGTAG104450.033.93586715
TCGTGTA104150.033.90139814
TGGTCGC97950.033.5913542
CGTCGTG107600.033.4625112
GTGGTCG101450.032.99622341
AGCGTCG107300.032.97458310
GTAGATC107400.031.9499731
GAGCGTC113500.031.2560049
AGAGCGT114750.030.9369358
GATCGGA116250.030.813351
TCTCGGT112550.030.71643436
TAGATCT112800.030.60352932
AAGAGCG114700.030.3194077
GTCGTGT120650.030.06720513
TGTAGGG122450.029.39793217
GTAGGGA119150.028.99498218
GTGTAGG127550.028.50225316
TCGGTGG123250.028.4897938
Produced by FastQC (version 0.11.2)