FastQCFastQC Report
Mon 31 Oct 2016
G142-M15-Index-1_ATCACG_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M15-Index-1_ATCACG_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17641715
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1467590.8318862423522884TruSeq Adapter, Index 1 (100% over 50bp)
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA460050.26077396670335057No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG382580.21686100245922801No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG329590.18682424016032453No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG278530.15788147580889952No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG256990.14567177850906218No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT255180.1446458011593544No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC250430.14195331916426493No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG241600.13694813684497226No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA235330.13339406061145417No Hit
GACGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGAC216330.12262413263109626No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG210340.11922877112570973No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG204500.11591843536753654No Hit
GGGGAACGACGCGCGGGTGGAGGGGTCGGGAGGAACGGGGAGCGGGAAAG176480.1000356257880824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT173100.039.2745540
CTCGTAT184350.038.68937742
TCTCGTA177800.038.31048641
ACGATCT177850.038.2002637
GATCTCG178200.038.1269639
TCACGAT186050.037.9460335
ATCACGA197600.037.57526834
CACGATC183450.037.04676436
TCGTATG202200.036.7946143
CATCACG205650.036.2979233
CGATCTC194450.034.87136538
CGTATGC220000.033.90696744
ACATCAC231950.032.40925232
ACGTCTG234950.032.18303315
CGTCTGA235500.032.06102816
GATCGGA246450.030.7014391
GCACACG249450.030.45399911
GTCACAT248550.030.44817729
ATCGGAA248350.030.4467322
ACACGTC249100.030.42575313
Produced by FastQC (version 0.11.2)