FastQCFastQC Report
Mon 31 Oct 2016
G142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16298935
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC334910.2054796831817539No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA293660.18017128112971798No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG235600.1445493217808403No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC196630.12063978413313509No Hit
AAAAAATCCTCCGAATGATTATAACCTAGACTTACAAGTCAAAGTAAAAT196420.12051094135905199No Hit
CTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATAG171410.10516638050277519No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG169750.10414790905049931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC68600.017.620681
GGCCTTA89800.016.2767664
CCTTATA91550.016.0162096
TGGCGCA74200.015.6998224
GCCTTAT94700.015.5516665
GGGCGAT14100.013.872511
GGCGATC14300.013.3713932
GGTGGCG92700.013.1822192
GTGGCGC91350.013.0645383
GGTAAAA117550.012.49442923
AATTAGA116400.012.39566716
GGACGAG45450.012.3791311
CCTGGCC125150.012.3103751
ATTACAC119450.012.25731229
GAGGTAA120600.012.2504221
AGAGGTA121000.012.02377720
GCGCACG98700.011.9382266
GCCGGGT66000.011.8879891
AGGTAAA125700.011.70184622
CTCCATA123800.011.66896944
Produced by FastQC (version 0.11.2)