FastQCFastQC Report
Mon 31 Oct 2016
G142-M12-Index-25_ACTGAT_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M12-Index-25_ACTGAT_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15772452
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC269620.17094361739062514No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC263770.1672346189419375No Hit
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG247690.1570396283342628No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC244040.15472546690901326No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC239710.1519801740401556No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA230910.14640082594640327No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT214400.1359332081023293No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC204840.12987200721866202No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG196270.12443848299554185No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC181630.11515647662139025No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC175740.11142211749954921No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG174640.11072469898783019Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCC171760.10889873052078394No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG169630.10754827467536437No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC168810.10702838087571925No Hit
CTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC167010.10588715058381537No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC161140.10216547179855104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG34300.028.14389415
GATCTCG30850.028.02945534
ATCTCGG31150.028.02833435
TCGTGTA35250.027.0707514
CGTCGTG37250.026.01056312
GTGGTCG37400.024.19950341
TCTCGGT37700.023.91741636
AGAGCGT40300.023.8376438
GTAGATC37700.023.76263631
TGGTCGC38550.023.47789842
TAGATCT38600.023.25710532
AAGAGCG40800.023.0072987
GATCGGA43350.022.9639781
GTCGTGT43700.022.4271213
GTAGGGA43100.022.15936718
GAGTGTA43300.021.81801627
TGTAGGG45500.021.6673617
ATCGGAA47200.020.7656752
GTGTAGG47900.020.52476716
AGCGTCG48950.020.3274910
Produced by FastQC (version 0.11.2)