FastQCFastQC Report
Mon 31 Oct 2016
G142-M11-Index-23_GAGTGG_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M11-Index-23_GAGTGG_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9849514
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA147410.14966220668349728No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT104830.10643164728736869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG12600.032.07027441
ACGAGTG15100.028.94513732
CACGAGT16100.027.01115831
GATCGGA16900.026.9996511
GTCACGA16250.026.8968529
ATCTCGT15400.026.6671342
CGAGTGG16700.026.30386733
TCACGAG16450.026.16941830
AGTCACG17450.025.42803228
ATCGGAA18950.024.1938132
CGTCTGA19850.023.3528616
ACGTCTG19800.023.0773815
TCTCGTA21450.020.78365343
CTCGTAT21150.020.5591844
GGATATC21850.018.79504438
GCACACG25850.018.35886811
TCGGAAG27050.017.1934553
GATATCT24250.017.02616739
ACACGTC27200.016.88012913
CGGAAGA29100.016.3598754
Produced by FastQC (version 0.11.2)