FastQCFastQC Report
Mon 31 Oct 2016
G141-M15-Index-14_AGTTCC_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M15-Index-14_AGTTCC_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22834646
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG363400.15914413562618837No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG356440.15609613567033181No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT273640.11983544654031422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG14950.019.26504941
TCCGTAT17550.017.51342437
CCGTATC17500.016.21232238
GAGTCGC74950.016.0647566
GTTCCGT22200.015.589210535
TTCCGTA22500.014.90217136
GTCGGCT43500.014.8347191
AGAGTCG82550.014.5004355
CGTATCT19650.014.328979539
GTCGGAT13150.014.2787731
AGTCGCC87600.014.0239867
ATCTCGT20900.013.78252442
GTCCAGA100400.013.7791271
TATCCGA66600.013.16725638
TTATCCG67200.013.04977837
GCCGACA62600.012.6873481
ACGTCTG31300.012.59863615
CGTCTGA31950.012.34232516
ATCGCCG65750.012.139977
TCGGCTG51200.012.09777452
Produced by FastQC (version 0.11.2)