FastQCFastQC Report
Mon 31 Oct 2016
G141-M14-Index-13_AGTCAA_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M14-Index-13_AGTCAA_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13547832
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG895120.6607108797924273Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC124400.041.3519342
ATCTCGG143250.038.55644235
GATCTCG148150.037.3433534
GTCGCCG139400.037.25190444
TCGTGTA159500.035.56927514
GTAGATC160150.034.941231
CTCGGTG159050.034.9127537
CGTCGTG169700.034.80451212
CGTGTAG164400.034.77125515
TCGGTGG168050.034.2464838
TCTCGGT163550.033.85334436
GTGGTCG156400.033.81670441
AGCGTCG172550.033.51382410
GAGCGTC172550.033.4448669
AGAGCGT173850.033.3598488
TAGATCT168500.033.2158732
GCGTCGT177250.033.207911
GATCGGA185300.033.0871431
ATCGGAA183350.033.01942
GAGTGTA171250.032.74076527
Produced by FastQC (version 0.11.2)