FastQCFastQC Report
Mon 31 Oct 2016
G140-M15-Index-8_ACTTGA_BC9WPLANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M15-Index-8_ACTTGA_BC9WPLANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23981746
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1146240.4779635310956925TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG169600.042.7583439
ATCTCGT170950.042.36540240
CGTATGC212600.038.7725544
GATCGGA208850.038.572871
TCGTATG215050.038.28410743
ATCGGAA211200.038.1980132
CGTCTGA213100.038.09131216
TCTCGTA197400.038.0067341
TCGGAAG219600.036.823223
CTCGTAT223800.035.5427342
CGGAAGA233150.034.795314
ACACGTC246500.034.3370713
TTGAATC213750.034.1536
ACGTCTG250700.033.7163415
CACGTCT253800.033.45581414
GAACTCC246300.033.21407321
GAATCTC220300.033.15632638
GCACACG264450.031.985511
CACACGT265350.031.96793612
ACTTGAA263850.030.900834
Produced by FastQC (version 0.11.2)