FastQCFastQC Report
Mon 31 Oct 2016
G139-M14-Index-9_GATCAG_BC9WPLANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M14-Index-9_GATCAG_BC9WPLANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171777
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG171430.1301494855249979Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC30250.030.97826842
ATCTCGG38750.025.81160735
GATCTCG43200.023.2514934
TCGTGTA48800.020.35986314
CGTGTAG49550.020.27674115
CTCGGTG50450.019.95800437
TCGGTGG51600.019.85190438
CGTCGTG52150.019.59372712
GTAGATC53450.019.04090131
TCTCGGT53650.018.96869536
GTGGTCG50600.018.77863141
AGAGCGT55750.018.2141768
AAGAGCG56400.017.7330277
GAGCGTC62000.016.9041469
GATCGGA54700.016.841741
TAGATCT61050.016.70567532
ATCGGAA54150.016.5558872
AGCGTCG64400.016.34049810
GAGTGTA63600.015.83736527
GTCGTGT66100.015.65844513
Produced by FastQC (version 0.11.2)