FastQCFastQC Report
Mon 31 Oct 2016
G139-M14-Index-9_GATCAG_BC9WPLANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG139-M14-Index-9_GATCAG_BC9WPLANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171777
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC136660.10375213610130204TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC31900.034.3623744
TCGTATG32350.033.6885143
TCTCGTA31250.032.1039341
GATCTCG33600.029.66998739
ATCTCGT34350.029.45258540
TCACGAT44800.024.47630530
CACGATC44850.024.35517331
ACGATCA45400.024.29317732
GTCACGA45150.024.2867529
CTCGTAT43550.023.7153142
CGATCAG47250.022.98323633
AGTCACG49150.022.69743728
ACACGTC58900.018.97346313
GATCGGA54900.018.900451
ACGTCTG61050.018.44751715
ACGTTCG3600.018.1363266
CGTCTGA51750.017.85204516
ATCGGAA58700.017.674382
CACGTCT65150.017.22172214
GTCGGAT7800.017.1167371
Produced by FastQC (version 0.11.2)