FastQCFastQC Report
Mon 31 Oct 2016
G138-M13-Index-4_TGACCA_BC9WPLANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M13-Index-4_TGACCA_BC9WPLANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14295611
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG619950.4336645701957055Illumina Single End PCR Primer 1 (100% over 50bp)
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT241740.16910085200275807No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC232830.16286816981799518No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC220640.15434107713199527No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC204350.14294597131944903No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC197790.1383571503169749No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC180330.12614361149026787No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC176180.12324062259388563No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC172780.12086227024504234No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC168730.11802923288833196No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC162770.113860121123889No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA162570.11372021804454528No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG159980.11190847316704405No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC159460.11154472516075038No Hit
CCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCC150700.10541697028549532No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG150550.10531204297598754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG88450.040.0126434
CGTGTAG93650.039.06632615
ATCTCGG91500.038.63094335
TCGTGTA94400.038.41985714
CGTCGTG97700.038.09667212
TGGTCGC91750.037.26708242
GTGGTCG93700.037.24281741
AGAGCGT99350.037.018958
GTAGATC97450.036.7501231
TCTCGGT98450.036.64380636
AAGAGCG99400.036.579237
GATCGGA103300.036.5652771
GTCGTGT102450.036.53000613
TAGATCT100650.035.70796632
GTAGGGA99100.035.69877218
AGCGTCG103750.035.56300410
TGTAGGG105200.035.45508617
GAGCGTC104700.035.1595159
CTCGGTG102450.035.10685337
TCGGTGG105200.034.71324538
Produced by FastQC (version 0.11.2)