G138-M11-Index-2_CGATGT_BC9WPLANXX_L002_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G138-M11-Index-2_CGATGT_BC9WPLANXX_L002_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16919179
Sequences flagged as poor quality	0
Sequence length	50
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	49312	0.29145622255075143	Illumina Single End PCR Primer 1 (100% over 50bp)
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC	27063	0.15995456989963874	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	26735	0.15801594155366522	No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG	21768	0.1286587251071698	No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG	20657	0.12209221263041192	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	18881	0.11159524939123819	No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC	17915	0.10588575249425519	No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT	17153	0.10138198786123133	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTGTA	7195	39.00605	14
CGTGTAG	7380	38.424442	15
GATCGGA	7845	37.64978	1
AGAGCGT	7575	36.99638	8
AAGAGCG	7680	36.375355	7
GATCTCG	7510	35.586113	34
ATCTCGG	7670	34.786617	35
TGTAGGG	8335	34.742847	17
GTCGTGT	8400	34.371815	13
GAGTGTA	8135	34.251427	27
GTAGATC	7835	34.19855	31
TAGATCT	7900	33.911354	32
GTAGGGA	8240	33.616653	18
ATCGGAA	8655	33.561687	2
GTGGTCG	7640	33.464787	41
GTGTAGG	8675	33.353233	16
TCTCGGT	8220	32.461544	36
CTCGGTG	8205	32.254215	37
CGTCGTG	8905	32.167953	12
AGTGTAG	8695	31.967638	28