G138-M10-Index-1_ATCACG_BC9WPLANXX_L002_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G138-M10-Index-1_ATCACG_BC9WPLANXX_L002_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16446845
Sequences flagged as poor quality	0
Sequence length	50
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	136357	0.8290769445446832	Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	38932	0.23671409318930164	No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT	29439	0.17899481633103492	No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC	25172	0.15305063068327085	No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG	22445	0.13646994301946666	No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	19740	0.12002301961257615	No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC	16825	0.1022992555715093	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTGTA	18485	42.09401	14
GATCGGA	19210	41.661728	1
CGTGTAG	18805	41.599617	15
AGAGCGT	18825	41.363663	8
GATCTCG	18465	41.063744	34
GTCGTGT	19720	40.709244	13
TGTAGGG	19615	40.46483	17
AAGAGCG	19035	40.40907	7
GAGTGTA	19215	40.124737	27
GTGGTCG	18455	40.083218	41
GTGTAGG	19800	40.062206	16
GTAGATC	19170	39.98155	31
TCTCGGT	19300	39.653194	36
TAGATCT	19385	39.612724	32
ATCTCGG	19150	39.60637	35
GTAGGGA	19150	39.569576	18
ATCGGAA	20030	39.476746	2
TCGGAAG	20275	39.205544	3
AGTGTAG	19780	39.087498	28
CTCGGTG	19480	39.03942	37