FastQCFastQC Report
Mon 31 Oct 2016
G137-M12-Index-16_CCGTCC_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M12-Index-16_CCGTCC_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616783
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1164640.8552974663692592Illumina Single End PCR Primer 1 (100% over 50bp)
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC264760.1944365273354213No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC238170.1749091543869062No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC219640.16130094751454876No Hit
CTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC214180.15729118985005489No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC212660.15617492031708222No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG196760.14449815349190775No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC195450.14353610540756948No Hit
GGAGGATTCAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCG184570.13554596559260731No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC181500.1332913948911428No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT180770.13275529176017567No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT172770.12688018895505643No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC166780.12248120572972339No Hit
CCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCC165840.12179088115012188No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC157810.11589374670948345No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC151520.11127444712895843No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG145380.10676530572602942No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG137800.10119864581817893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCG184950.038.6359641
ATCTCGG189450.038.19229535
GATCTCG189300.038.18857634
TGGTCGC189400.037.48198342
GGTCGCC188350.037.27059643
TAGATCT196500.037.0243432
TCTCGGT198250.036.80529836
TCGGTGG201200.036.5666738
GTAGATC199050.036.45139731
CTCGGTG201100.036.10636537
GAGTGTA203000.035.7486627
CGTGTAG181450.035.72990815
AGTGTAG203100.035.60371428
TCGTGTA182100.035.5456214
TGTAGAT205600.035.5360130
GTAGGGA177050.035.53258518
AGATCTC204100.035.36971733
TGTAGGG186950.035.1030317
GTGTAGG188450.034.77778216
TAGGGAA179950.034.72327819
Produced by FastQC (version 0.11.2)