FastQCFastQC Report
Mon 31 Oct 2016
G137-M11-Index-15_ATGTCA_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M11-Index-15_ATGTCA_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823097
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4449203.218670895530864Illumina Single End PCR Primer 1 (100% over 50bp)
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC172450.12475496627130664No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC169560.12266426257444335No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC166850.12070377571683105No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC151400.10952683034778675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG599550.041.747493
AGAGCGT595600.041.6823738
GTAGGGA568000.041.6299618
GATCGGA597650.041.59831
AAGAGCG591950.041.409717
ATCGGAA598100.041.381662
GTGGTCG644700.041.21558841
TCGTGTA589750.041.20954514
ATCTCGG651550.041.19579735
CGTGTAG589750.041.17805515
GATCTCG651650.041.1728734
TAGGGAA572000.041.1608119
TGGTCGC641100.041.0582542
TGTAGGG596600.041.04644417
TCTCGGT660500.040.9209436
GTGTAGG599550.040.82261316
TAGATCT665150.040.74697532
GTCGTGT607750.040.7348613
TCGGTGG667250.040.7224938
CTCGGTG660650.040.7102437
Produced by FastQC (version 0.11.2)