FastQCFastQC Report
Mon 11 Jan 2021
G119-M5_ATGTCA-_AC6PMVANXX_L004_001.R_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M5_ATGTCA-_AC6PMVANXX_L004_001.R_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850804
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT11948035.730249058981131TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT219070.10506549291816278TruSeq Adapter, Index 15 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)