FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_WT_Rep2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_WT_Rep2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67091313
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3036750.4526293888450208No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2751040.4100441438670309No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC1649990.24593198824414123No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1421320.21184858910124477No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1375030.20494903714285634No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1115540.16627189871809483No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1111050.1656026615547083No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA1084020.16157382402100254No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1073870.16006096050020663No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG901090.13430799901024443No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC853070.12715058952565142No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG726450.10827780341696397No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG725620.10815409142462304No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA723320.10781127506030476No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT716410.10678133546141808No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC705370.10513581691269033TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG125000.026.84203339
ATCTCGT124450.026.81912840
CGTATGC148250.022.71896244
GTGATTA156600.021.0129011
CGTTATG37650.020.5117153
TCGTATG171400.019.68902443
GATCGGA175300.019.1354921
TCGTTAT42650.019.0872272
CTCGTAT188600.018.43125342
ATCGGAA187650.017.8805732
CCCTACG286100.017.58627324
TCTCGTA192650.017.5303341
TACGTTC294200.017.1624727
CCTACGT296100.016.98501425
GCCTATA196850.016.90754310
TATCCGA194950.016.75927538
CGTCTGA202000.016.68345316
CTACGTT303750.016.63008126
TACCCTA306000.016.56440522
ACCCTAC305350.016.56363123
Produced by FastQC (version 0.11.2)