FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_NIPBL_Rep2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_NIPBL_Rep2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60024086
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC4927100.820853815250098No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA4218220.7027545575621092No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA3222130.536806174774573No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC3039040.5063034195972597No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT2168690.36130329414761936No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1915410.31910689985350216No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1866960.3110351401269151No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1845400.3074432486985308No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT1679400.2797876838974275No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG1569110.26141339328348956No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG1481110.24675261194314563No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG1468760.24469510456185872No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1406060.23424929785686366No Hit
GCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGG1354740.2256993967388358No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1251550.20850796461940294No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1112590.18535725808469622No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1075270.1791397539980867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1029920.1715844536141708TruSeq Adapter, Index 5 (100% over 50bp)
CTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGG982700.16371761162677262No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC967530.16119029284344288No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA914380.15233551411345106No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG847120.14113001237536546No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT820850.13675343594569686No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA820660.13672178198598475No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA813330.13550060554024929No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT781060.13012443038282998No Hit
CCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT756270.1259944216393399No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG741720.12357039472454441No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC720810.12008679315833314No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA686330.11434243246952565No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG664760.1107488750432618No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC648830.10809494042108364No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT641340.1068471080092748No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC626110.10430979323866757No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT624640.10406489155036862No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA623980.10395493569031605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT151600.031.69644540
CGTATGC156200.030.95646344
GATCTCG166450.029.00084539
AGTGATC177500.027.12095636
TCGTATG202150.024.00689743
GATCGGA203350.023.8233241
ATCGGAA219000.022.0516642
CGTCTGA221650.021.83399416
TCGTTAT35850.021.3570382
TCTCGTA228050.021.1478141
CGTTATG36050.020.872343
ACGTCTG235150.020.66465815
GTGATCT238950.020.35834337
GTGATTA94950.019.7038211
TCGGAAG251600.019.439233
GACGGGG358400.019.3757211
CTCGTAT261050.019.37551342
CCGACTA182700.019.31274616
CGACTAT182900.019.26767217
CGGCTAT33450.019.1479971
Produced by FastQC (version 0.11.2)