FastQCFastQC Report
Mon 6 Mar 2017
G151-M36-Index-1_ATCACG-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M36-Index-1_ATCACG-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29172999
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG13700.033.40973712
AGCGTCG14650.031.69407710
GCGTCGT14800.031.5215411
GTCGCCG12200.028.31353844
TCGTGTA21400.021.90194514
GTCGTGT21600.021.69922413
GATCGGA22250.020.6663911
GAGCGTC22800.020.6542059
CGTGTAG24200.019.64021915
TGGTCGC17200.019.5681442
ATCGGAA24950.018.9595242
GATCTCG21600.018.02501134
GTGGTCG19250.017.59681941
AGAGCGT26650.017.5877578
GGTCGCC21650.016.1575343
AAGAGCG30000.016.1370837
ATCTCGG24900.015.72422635
TCGGAAG32850.014.4002273
TCGGTGG29500.013.49556338
TCTCGGT28200.013.2599936
Produced by FastQC (version 0.11.2)