FastQCFastQC Report
Mon 6 Mar 2017
G151-M35-Index-27_ATTCCT-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M35-Index-27_ATTCCT-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28457132
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG19850.036.80396312
GTCGCCG16650.035.01775744
GCGTCGT21100.034.51964611
AGCGTCG21250.034.3795310
GTCGTGT27000.027.38329113
GAGCGTC27000.027.0579669
TGGTCGC21000.027.02674542
TCGTGTA27800.026.43688414
CGTGTAG28400.025.95551915
GATCTCG24600.025.66294934
GTGGTCG22750.025.6223941
GATCGGA29400.024.770431
GGTCGCC24100.024.19161843
ATCGGAA31900.022.9673162
AGAGCGT32250.022.926078
ATCTCGG30100.021.85007935
AAGAGCG35350.021.3511737
TCTCGGT30850.020.03539536
TCGGTGG31950.019.96538
TCGGAAG38900.019.0043283
Produced by FastQC (version 0.11.2)