FastQCFastQC Report
Mon 6 Mar 2017
G151-M33-Index-23_GAGTGG-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M33-Index-23_GAGTGG-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28146953
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT30900.031.95810944
TCTCGTA29050.031.72265643
GTCACGA31850.031.07498429
ACGAGTG33000.029.92616532
CGAGTGG33850.029.23956733
CGTCTGA35100.028.58730316
TATCTCG32950.027.96857541
TCACGAG35700.027.72425330
CACGAGT35950.027.53165231
ACACGTC37200.027.0327513
GATCGGA37800.026.3814031
ATCTCGT35250.026.20555542
ACGTCTG39250.025.67691215
ATCGGAA39850.025.2250022
AGTCACG41800.024.1521128
TCGGAAG45350.022.3578433
CACGTCT48500.021.14273514
CGGAAGA48750.020.7555124
CACACGT55500.018.71397212
GCACACG56100.018.23899511
Produced by FastQC (version 0.11.2)