FastQCFastQC Report
Mon 6 Mar 2017
G151-M32-Index-22_CGTACG-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M32-Index-22_CGTACG-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26067760
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG21550.038.47830233
CGTACGT21800.038.2380234
ACCGTAC24400.034.43462832
CGTAATC23000.033.9481238
GTACGTA25050.033.1016535
CTCGTAT25950.031.69849244
TCTCGTA25000.031.40804743
TACGTAA26850.030.39104736
CACCGTA28150.029.69115331
GATCGGA29650.028.435671
CGTCTGA29600.028.39810416
AATCTCG28350.027.85233341
ATCGGAA30350.027.757552
ACGTAAT28800.027.26424237
GTCACCG31900.026.61389729
ACACGTC32700.025.84051513
TCACCGT33850.024.9511830
ACGTCTG34100.024.650615
ATCTCGT32200.024.38522142
TCGGAAG35550.023.757243
Produced by FastQC (version 0.11.2)