FastQCFastQC Report
Mon 6 Mar 2017
G151-M31-Index-21_GTTTCG-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M31-Index-21_GTTTCG-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102330
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG18200.038.1890833
TCTCGTA22250.029.85293643
CTCGTAT23050.029.77005644
GTTTCGG24300.028.78327434
TCGGAAT24050.027.8016937
TTTCGGA25200.027.49345435
CGTCTGA27550.026.27786316
CGGAATC24950.026.1816938
AATCTCG25800.025.83086241
ATCGGAA29600.024.1514152
GATCGGA29500.024.027641
ACGTCTG30350.023.85368315
ATCTCGT28700.023.6803242
ACACGTC31550.023.15564713
TTCGGAA30400.023.0076836
AGTCACG32850.021.82716628
GTCACGT33350.021.23583629
TCGGAAG36000.019.9780433
ACGTTTC36550.019.19670932
CGGAAGA38350.018.6406654
Produced by FastQC (version 0.11.2)