FastQCFastQC Report
Mon 6 Mar 2017
G151-M29-Index-19_GTGAAA-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M29-Index-19_GTGAAA-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27759577
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24550.030.45958944
AACGATC23450.029.82653638
TCTCGTA23500.029.57545943
CGATCTC25650.027.26880540
GATCTCG26150.026.83152641
CGTCTGA30400.025.91372716
ACGATCT28550.025.3464439
TGAAACG31500.024.57832135
GAAACGA32700.023.6090636
GATCGGA33300.023.3348581
AAACGAT30900.022.99133337
ACACGTC34450.022.86751413
ACGTCTG34450.022.73976315
AGTCACG35000.022.49766328
ATCTCGT31500.022.41347142
ATCGGAA35750.021.8411122
GTCACGT38050.020.6936729
TCGGAAG40400.019.489713
CGGAAGA41550.019.216824
CACGTCT45250.017.70141414
Produced by FastQC (version 0.11.2)