FastQCFastQC Report
Mon 6 Mar 2017
G151-M26-Index-15_ATGTCA-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M26-Index-15_ATGTCA-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27659597
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG24600.037.30254412
GTCGCCG18650.037.16161744
GCGTCGT25150.036.13678711
AGCGTCG26050.035.0572510
GTCGTGT31100.029.85879513
GAGCGTC31200.029.5526319
TCGTGTA32200.028.63355314
TGGTCGC23400.028.48482142
GATCTCG26450.027.69304734
CGTGTAG34000.027.63516815
GATCGGA33850.027.2986351
GTGGTCG25950.026.61552241
GGTCGCC26900.026.00857243
AGAGCGT36100.025.7242058
ATCGGAA36350.025.4817222
AAGAGCG38200.024.2523547
TCTCGGT32050.023.12801236
ATCTCGG33850.022.35322235
TCGGTGG35550.022.08808138
TCGGAAG43100.021.7469483
Produced by FastQC (version 0.11.2)