FastQCFastQC Report
Mon 6 Mar 2017
G151-M25-Index-11_GGCTAC-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M25-Index-11_GGCTAC-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24281262
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG12450.035.70335812
GCGTCGT13500.032.7636311
GTCGCCG10300.031.82757844
AGCGTCG14300.031.39235310
GTCGTGT18700.024.35834113
GAGCGTC19050.023.5648149
CGTGTAG20850.022.37389815
TCGTGTA21150.021.95267914
GATCGGA20300.021.7851641
TGGTCGC15150.021.63507542
GATCTCG17400.020.732234
GTGGTCG17300.019.32568241
ATCGGAA22900.019.3119772
GGTCGCC17300.019.20316743
AAGAGCG24000.019.1628997
AGAGCGT23700.018.94138
TCGGTGG23250.016.74485638
TCTCGGT22600.016.44816436
TCGGAAG27700.016.2837243
ATCTCGG24100.015.6984235
Produced by FastQC (version 0.11.2)