FastQCFastQC Report
Mon 6 Mar 2017
G151-M23-Index-9_GATCAG-_BCALRAANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M23-Index-9_GATCAG-_BCALRAANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28079344
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15100.025.32411642
TCTCGTA15400.023.8318541
GATCGGA17200.023.4362931
TCGTATG17250.022.04002443
CGATCAG17700.021.98125633
ACGATCA18700.020.57069432
CGTATGC18650.020.50308844
GTCACGA19600.019.9601529
ATCGGAA19750.019.9491522
CACGATC20600.018.88653831
GATCTCG21050.017.95731539
ACACGTC23750.017.42174713
CGTCTGA23500.017.13908616
TCACGAT23250.016.92184630
ATCTCGT23400.016.3416340
ACGTCTG26450.015.72677915
AGTCACG26850.014.81618928
TCGGAAG28800.014.1389953
CGGAAGA30600.013.381444
CACGTCT36800.011.18400714
Produced by FastQC (version 0.11.2)