FastQCFastQC Report
Mon 6 Mar 2017
G151-M22-Index-8_ACTTGA-_BCALRAANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M22-Index-8_ACTTGA-_BCALRAANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26520906
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4900.016.16651712
AGCGTCG5650.015.18889510
GCGTCGT5850.014.29347211
GTCGCCG4050.013.58123744
GAGCGTC11850.08.3558279
GTCGTGT11150.07.30191713
GATCTCG11350.06.97700834
TCGTGTA13350.06.593086214
CGTGTAG13400.06.075791415
TGGTCGC9553.79805E-95.989456742
AGAGCGT15700.05.8863018
GTGGTCG11451.2660166E-95.571460241
GATCGGA15400.05.428671
GGTCGCC11152.224624E-85.327561443
ATCGGAA16950.05.3217512
ATCTCGG15801.8189894E-125.29032735
AAGAGCG19000.05.2113387
Produced by FastQC (version 0.11.2)