FastQCFastQC Report
Mon 6 Mar 2017
G151-M21-Index-7_CAGATC-_BCALRAANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M21-Index-7_CAGATC-_BCALRAANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28082907
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5900.020.88634312
AGCGTCG6100.019.84073310
GCGTCGT7450.016.83617611
GTCGCCG5550.016.64968944
GAGCGTC11650.010.9549379
TCGTGTA12800.010.65877714
GTCGTGT12200.010.46149513
TGGTCGC9800.09.652719542
GTGGTCG11750.08.79895241
CGTGTAG15300.08.4855215
AGAGCGT17200.08.1875658
GGTCGCC12650.08.17414743
ATCGGAA16950.07.78824952
GATCGGA16750.07.6183731
GATCTCG14050.07.358134334
AAGAGCG19250.06.74405967
ATCTCGG20100.06.23767435
TCGGAAG23150.06.1779433
CGGTGGT18900.05.70260839
CGGAAGA26000.05.6702224
Produced by FastQC (version 0.11.2)