FastQCFastQC Report
Mon 6 Mar 2017
G151-M20-Index-6_GCCAAT-_BCALRAANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M20-Index-6_GCCAAT-_BCALRAANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24567347
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5000.022.0043112
AGCGTCG5450.020.59114610
GCGTCGT5850.019.18320511
GTCGCCG4800.017.41770244
GAGCGTC10600.011.2093559
GTCGTGT10900.010.49746913
GATCGGA11550.09.3336781
TCGTGTA12750.08.97430614
CGTGTAG13250.08.96764815
GGTCGCC9950.08.84442243
TGGTCGC9250.08.79982542
GATCTCG10150.08.66877234
ATCGGAA13700.08.0298542
AGAGCGT15650.07.8734428
GTGGTCG11100.07.72897741
AAGAGCG17650.06.7318817
TCGGAAG18600.06.62442733
ATCTCGG15300.06.469629335
TCGGTGG16450.06.418370738
TCTCGGT15350.05.87536336
Produced by FastQC (version 0.11.2)