FastQCFastQC Report
Mon 6 Mar 2017
G151-M18-Index-4_TGACCA-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M18-Index-4_TGACCA-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23853131
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9200.015.28142643
CGTATGC10200.013.99990144
TCTCGTA9100.013.75885941
GATCGGA10750.012.9155191
CTCGTAT10600.012.8478742
CGTCTGA13050.011.30547616
ATCGGAA13200.010.6772152
AATCTCG13350.010.20164839
ACACGTC15400.09.72354513
ACGTCTG16150.09.40760915
ATCTCGT14400.08.847486540
TCGGAAG18650.08.0292963
CGGAAGA21750.06.5815334
CACGTCT25300.06.52768114
GCACACG29550.06.110353511
CACACGT31950.05.582757512
Produced by FastQC (version 0.11.2)