FastQCFastQC Report
Mon 6 Mar 2017
G151-M16-Index-2_CGATGT-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M16-Index-2_CGATGT-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25262654
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG6050.029.09471544
CGTCGTG7850.027.19015312
AGCGTCG8250.026.67205810
GCGTCGT8100.026.62266311
TGGTCGC10500.016.5517342
TCGTGTA13500.016.299314
GAGCGTC13850.016.0464699
GTCGTGT13750.015.52295213
GGTCGCC11900.014.60634643
CGTGTAG15700.014.15541815
GATCTCG13300.014.05828934
GTGGTCG13750.012.79830541
GATCGGA17850.012.4486251
AGAGCGT18500.012.0129298
ATCGGAA19950.011.3588492
AAGAGCG21050.010.5577667
ATCTCGG17700.010.43915135
TCGGTGG18900.010.357985538
TCTCGGT18750.09.97177636
TCGGAAG24650.09.1938643
Produced by FastQC (version 0.11.2)