FastQCFastQC Report
Mon 6 Mar 2017
G151-M13-Index-25_ACTGAT-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M13-Index-25_ACTGAT-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25993596
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10600.016.58221244
TCTCGTA11050.015.70687343
CGTCTGA15200.012.892632516
GATCGGA15850.011.5401151
ATCGGAA16800.011.4035572
TATCTCG15150.011.31063241
ACACGTC18300.010.8287913
ACGTCTG19100.010.02886615
ATCTCGT17200.09.834722542
TCGGAAG21750.08.6055943
CGGAAGA24050.08.0577674
CACGTCT30500.06.93019814
GCACACG33600.06.028583511
CACACGT33250.06.026114512
AGATCGG14651.70985E-105.09848542
Produced by FastQC (version 0.11.2)